Look at your start G Code ( Preferences/Printer/Machine Settings )
there should be the Gcode for the prime line.
in my case:
...
G1 X0.1 Y20 Z0.3 F5000.0 ; Move to start position
G1 X0.1 Y200.0 Z0.3 F1500.0 E15 ; Draw the first line
G1 X0.4 Y200.0 Z0.3 F5000.0 ; Move to side a little
G1 X0.4 Y20 Z0.3 F1500.0 E30 ; Draw the second line
...
and if you are Printing at a layer Hight of only 0.2mm and the prime line is at 0.3 it will show up as the second layer...
Great answer - it looks like you tried to use a code block, but it didn't render since it's not quite the right characters. Use triple 'backticks', like ``` and ```