No connection between Octoprint and Printer

What is the problem?
I just moved a few days ago and yesterday I finally had time to set up my printer again. After I did all the calibrations I took the SD-Card out of my Pi Zero 2W and changed the network. I plugged the Pi Zero 2W back into my Prusa I3MK3S+ (over GPIO) and opened the Webinterface.
Everything looked fine I updated to the latest version (1.8.6 ) and then tried to connect to my printer

Serial Port I used:


Printer Profile:
My old printer Profile

After I few seconds I get following errors:

Popup top left:
Could not autodetect your printer
No working connection parameters could be found.

Printer state:
Offline after error
No more candidates to test, and no working port/baudrate combination detected.

I once got another error but can't recreate it and do not rember the error message.

What did I already try to solve it?
I checked my GPIO pins, they look fine
I checked my Printersettings, RPI port is ON
I connected via SSH and checked the settings
Also tried /dev/ttyS0 as Serial Port

Have I tried running in safe mode?

Did running in safe mode solve the problem?

Systeminfo Bundle (62.5 KB)

Additional information about my setup
Browser: Chrome
Printer: Prusa I3MK3S+
OctoprintVersion 1.8.6 running on Raspberry Pi Zero 2 Rev 1.0

I hope you can help me. Thanks in advance

Have you checked the serial port setting in Prusa firmware?