Prusa i3mk3s mmu2s not connecting

What is the problem?
I cannot connect to my Prusa i3mk3s mmu2s using my setup
What did you already try to solve it?
Tried safe mode,
Changing timeout
Logs ( octoprint.log , serial.log or output on terminal tab at a minimum , browser error console if UI issue ... no logs, no support! )
Connecting to: /dev/ttyACM0
Changing monitoring state from "Offline" to "Opening serial port"
Connected to: Serial<id=0x6ef21570, open=True>(port='/dev/ttyACM0', baudrate=115200, bytesize=8, parity='N', stopbits=1, timeout=10.0, xonxoff=False, rtscts=False, dsrdtr=False), starting monitor
Changing monitoring state from "Opening serial port" to "Connecting"
Send: N0 M110 N0 125
Recv: start
Send: N0 M110 N0
There was a timeout while trying to connect to the printer
Changing monitoring state from "Connecting" to "Offline"
Connection closed, closing down monitor

serial.log (1.0 KB)

octoprint (1).log (884.9 KB)
Additional information about your setup (OctoPrint version, OctoPi version, printer, firmware, browser, operating system, ... as much data as possible )

Octoprint version:1.4.0
Octopi version 0.15.0PE
Printer: Prusa i3 mk3s mmu2s
Raspberry pi: 3b+
Connection type: USB
Browser: Chrome, Chromium
OS: Windows, Linux

On the LCD check Settings->RPi port = OFF.
That disables the connection for a Pi Zero and should allow the USB connection to succeed and it works for me.